3D micropatterning silk hydrogels via three-photon absorption


Optical tools are great at delivering energy to a precise location in time and space. Here I took advantage of the large three-photon cross-section of silk to carve vessel-like structures. I seeded these structures with cells and demonstrated that they could take advantage of the voids created to penetrate deeply within the hydrogel. The goal for this work is to one day use these patterns to feed cells in artificial organs.